Additional file 1 of Hypoxia exposure blunts angiogenic signaling and upregulates the antioxidant system in endothelial cells derived from elephant seals ...

• Main Authors: Allen, Kaitlin N., Torres-Velarde, Julia María, Vazquez, Juan Manuel, Moreno-Santillán, Diana D., Sudmant, Peter H., Vázquez-Medina, José Pablo
• Format: Text
• Language: unknown
• Published: figshare 2024
• Subjects: Genetics; FOS: Biological sciences; Elephant Seals
• Online Access: https://dx.doi.org/10.6084/m9.figshare.25678610.v1; https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Hypoxia_exposure_blunts_angiogenic_signaling_and_upregulates_the_antioxidant_system_in_endothelial_cells_derived_from_elephant_seals/25678610/1

Additional file 1: Supplementary Figures S1-S6. Figure S1. Representative images for mitochondrial staining after 0, 1, or 6 h hypoxia exposure followed by 30 min reoxygenation. Upper: seal. Lower: human. MitoTracker Red CMXRos (red). Hoescht (blue). Scale bar is 50 µm. Figure S2. Uncropped western blots. (A) HIF-1α. (B) β-actin. Figure S3. Raw gap area for all species and conditions during scratch assay. Figure S4. Total within cluster sum of squares for clustering results from k=1...30). Upper: seal. Lower: human. Diminishing returns were observed after 6 clusters thus k=6 (pink) was selected as the optimal clustering. Figure S5. k-means clustering and KEGG pathway enrichment for human cells. k-means clustering of gene expression data for human cells exposed to 1% O2 for up to 6 h. (B) KEGG pathway enrichment for human clusters. Numbers on the right axis correspond to human cluster numbers. Figure S6. Long-term hypoxia exposure modulates transcription and translation in human cells. (A) Reactome pathway ...