Microsatellite dataset Cryptopygus - Foregrounding geodiversity in landscape ecology.xlsx ...

• Main Authors: Monsanto, Daniela, Hedding, David William, Durand, Sandra, Parbhu, Shilpa Pradeep, Adair, Matthew Grant, Emami-Khoyi, Arsalan, Teske, Peter, Jansen van Vuuren, Bettine
• Format: Dataset
• Language: unknown
• Published: figshare 2023
• Subjects: Population ecology; Molecular evolution; Humps; Antarc*; antarcticus; Cryptopygus antarcticus; Marion Island
• Online Access: https://dx.doi.org/10.6084/m9.figshare.23995446; https://figshare.com/articles/dataset/Microsatellite_dataset_Cryptopygus_-_Foregrounding_geodiversity_in_landscape_ecology_xlsx/23995446

Sampling:Transect sampling was conducted on eastern Marion Island at two sampling areas, namely Nellie Humps (NH) Skua Ridge (SR). The NH site included 24 sampling points separated from each other by a geographic distance ranging from 30 to 120 m, while the SR site comprised 21 sampling points separated by distances ranging from 60 and 190 m. Cryptopygus antarcticus travei specimens were collected by extracting them from sampled moss and/or ferns (approximately 10 cm 3 ) using Berlese-Tullgren funnels. Twenty individuals of C. a. travei from each sampling point were identified and sorted using a compound light microscope, and stored in absolute ethanol (Merck, South Africa).Microsatellite genotyping:Whole genomic DNA was extracted using the DNeasy ® Blood and Tissue Kit (Qiagen ® , Hilden, Germany), according to the manufacturer's recommendations, with minor modifications that included a longer digestion step (overnight, but not more than 20 hours) and a reduced final elution volume (75 μl instead of 100 ...