Additional file 3 of Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights ...

Additional file 3. Primers and detection oligo. Excel file listing the primers used for amplification and transcription reactions and as detection oligo. Reverse primers consist of the 20-mers complementary sequence linked to the 3’ end of the T7 sequence 5’-CGATTGAGGCCGGTAATACGACTCACTATAGGG-3’ [45]...

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Bibliographic Details
Main Authors: Poisson, William, Prunier, Julien, Carrier, Alexandra, Gilbert, Isabelle, Mastromonaco, Gabriela, Albert, Vicky, Taillon, Joëlle, Bourret, Vincent, Droit, Arnaud, Côté, Steeve D., Robert, Claude
Format: Dataset
Language:unknown
Published: figshare 2023
Subjects:
Genetics
FOS Biological sciences
Rangifer tarandus
Online Access:https://dx.doi.org/10.6084/m9.figshare.22621945.v1
https://springernature.figshare.com/articles/dataset/Additional_file_3_of_Chromosome-level_assembly_of_the_Rangifer_tarandus_genome_and_validation_of_cervid_and_bovid_evolution_insights/22621945/1
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Summary:Additional file 3. Primers and detection oligo. Excel file listing the primers used for amplification and transcription reactions and as detection oligo. Reverse primers consist of the 20-mers complementary sequence linked to the 3’ end of the T7 sequence 5’-CGATTGAGGCCGGTAATACGACTCACTATAGGG-3’ [45]. Forward primer 20-mers are linked to the 3’ end of the adapter sequence. The resulting 53-mers reverse primer and 40-mers forward primer oligo were used for PCR. Forward primers were used also for RT-PCR. The fluorophore-linked homolog of the specific 20-mers adapter was the detector of probe binding. These 30 oligo were purchased from IDT as custom standard or HPLC-purified DNA. ...

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