Evolution, characterization, and expression profile of Egl-9 family hypoxia-inducible factor ( egln ) in rainbow trout ( Oncorhynchus mykiss ) under hypoxia stress ...
Egl-9 family hypoxia-inducible factor ( egln ), an oxygen-sensing enzyme family, has been thoroughly characterized in mammals and certain fishes, but there is few research on its involvement in reproductive development and hypoxic stress in rainbow trout. In this study, we investigated the gene stru...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | unknown |
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Taylor & Francis
2022
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6084/m9.figshare.19361887.v1 https://tandf.figshare.com/articles/dataset/Evolution_characterization_and_expression_profile_of_Egl-9_family_hypoxia-inducible_factor_i_egln_i_in_rainbow_trout_i_Oncorhynchus_mykiss_i_under_hypoxia_stress/19361887/1 |
| Summary: | Egl-9 family hypoxia-inducible factor ( egln ), an oxygen-sensing enzyme family, has been thoroughly characterized in mammals and certain fishes, but there is few research on its involvement in reproductive development and hypoxic stress in rainbow trout. In this study, we investigated the gene structure, physicochemical properties, and evolutionary connection of the egln gene family. The expression profile of egln gene family and their regulatory mechanism were explored using bioinformatics analysis and hypoxia treatment experiments. Five egln genes were discovered in the rainbow trout genome in this investigation ( egln1 , egln2a , egln2b , egln3a , and egln3b ). Domain prediction revealed that all egln proteins have p4hc conserved domains, and phylogenetic analysis revealed that rainbow trout egln2 and egln3 were closely related to Atlantic salmon . The results of real-time quantitative PCR (RT-qPCR) showed that egln genes were generally expressed in all detected tissues, and higher in the ovary, testis, ... |
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