BLASTN Analysis Results Files

BLASTN Analysis Results Files The Salmo salar soluble Toll-like receptor 5 (sTLR5) sequence was found in the Stephen et al. paper with a linked accession number. This accession number took us to the NIH website where we were able to download the CDS sequence of the sTLR5 as a FASTA file. The Salmo s...

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Bibliographic Details
Main Authors: Lofts, Brandon, Ramirez, Elizabeth, vasquez, delbert, Tang, Xiaolu
Format: Dataset
Language:unknown
Published: figshare 2022
Subjects:
Online Access:https://dx.doi.org/10.6084/m9.figshare.19333238
https://figshare.com/articles/dataset/BLASTN_Analysis_Results_Files/19333238
Description
Summary:BLASTN Analysis Results Files The Salmo salar soluble Toll-like receptor 5 (sTLR5) sequence was found in the Stephen et al. paper with a linked accession number. This accession number took us to the NIH website where we were able to download the CDS sequence of the sTLR5 as a FASTA file. The Salmo salar sTLR5 was then used to run a BLASTN analysis against each species cDNA on Ensembl Genome Browser 105. The search sensitivity was set to "Normal." We then identified hits with a high percent Identity and an e-value of 0.0, making sure that the hits were in fact different genes (different subject name and location in genome). These hits were then saved as a FASTA using their CDS. They were then uploaded into AliView and aligned using MUSCLE.