Additional file 1 of Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost ...

• Main Authors: Liang, Renxing, Li, Zhou, Lau Vetter, Maggie C. Y., Vishnivetskaya, Tatiana A., Zanina, Oksana G., Lloyd, Karen G., Pfiffner, Susan M., Rivkina, Elizaveta M., Wang, Wei, Wiggins, Jessica, Miller, Jennifer, Hettich, Robert L., Onstott, Tullis C.
• Format: Text
• Language: unknown
• Published: figshare 2021
• Subjects: Microbiology; FOS Biological sciences; Genetics; Geology; FOS Earth and related environmental sciences; East Siberian Sea; permafrost
• Online Access: https://dx.doi.org/10.6084/m9.figshare.14608585; https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Genomic_reconstruction_of_fossil_and_living_microorganisms_in_ancient_Siberian_permafrost/14608585

Additional file 1: Figure S1. Geographic location of the sampling site at Cape Chukochii near the East Siberian Sea coast. Figure S2. Image of the drilling site and the schematic of the sediment core (~22 m) from borehole Ch1-17. The red stars indicate the depth of the sediment samples (3.4, 5.8 and 14.8 m, meters below land surface) that were selected for metagenomic sequencing of iDNA and eDNA with and without DNA repair. Figure S3 Temperature and geochemistry profiles of the permafrost sediment collected at various depths from borehole Ch1-17. Figure S4. Concentration of low-molecular-weight organic acids in the permafrost sediment collected at various depths from borehole Ch1-17. Figure S5 Yield of iDNA and eDNA fractions from ancient permafrost sediment at 3.4, 5.8 and 14.8 m. The green bars represent the estimated cell numbers from the intracellular DNA fraction by assuming 2×10-15 g DNA/cell. Figure S6. Size distribution of DNA fragments in iDNA (3.4i, 5.8i and 14.8i) and eDNA (3.4e, 5.8e and 14.8e) ...