High-throughput sequencing of the kelp Alaria (Phaeophyceae) reveals epi-endobiotic associations, including a likely phaeophycean parasite ...
Whole genome sequencing datasets present the opportunity to not only study evolution in the target organism, but also the associated holobiont. The capacity to study epi-endobiotic kelp associations is improving substantially with the increased availability of high-throughput sequencing datasets. Th...
| Main Authors: | , , , , , , |
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| Format: | Text |
| Language: | unknown |
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Taylor & Francis
2021
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| Online Access: | https://dx.doi.org/10.6084/m9.figshare.14402189.v1 https://tandf.figshare.com/articles/journal_contribution/High-throughput_sequencing_of_the_kelp_i_Alaria_i_Phaeophyceae_reveals_epi-endobiotic_associations_including_a_likely_phaeophycean_parasite/14402189/1 |
| Summary: | Whole genome sequencing datasets present the opportunity to not only study evolution in the target organism, but also the associated holobiont. The capacity to study epi-endobiotic kelp associations is improving substantially with the increased availability of high-throughput sequencing datasets. The goal of this study was to determine if shotgun sequencing libraries could be used to document epi- and endophyte/faunal species colonizing Alaria kelp sporophytes from Kamchatka (Russia), the Bay of Fundy (Atlantic Canada) and Nuuk (Greenland). Mitochondrial cox I and plastid rbc L reads were extracted and assembled from six Alaria whole genome sequencing datasets. In total, contigs representing 11 epi-endobiotic species were assembled, of which Chordariacean diversity dominated. Given the presence of a newly discovered phaeophycean cox I sequence lacking an rbc L counterpart, we secondarily tested our hypothesis that the cox I sequence belonged to a phaeophycean parasite. The entire read dataset was assembled ... |
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