Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells
Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic kr...
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ftdatacite:10.6084/m9.figshare.14318646 2023-05-15T14:02:06+02:00 Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells Shanshan WANG Deqing ZHOU Haiyan LIN 2021 https://dx.doi.org/10.6084/m9.figshare.14318646 https://scielo.figshare.com/articles/dataset/Ameliorative_effects_of_phosphorylated_peptide_from_Antarctic_krill_Euphausia_superba_against_H2O2-induced_damage_in_MC3T3-E1_cells/14318646 unknown SciELO journals https://dx.doi.org/10.1590/fst.64920 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 90899 Food Sciences not elsewhere classified FOS Other engineering and technologies dataset Dataset 2021 ftdatacite https://doi.org/10.6084/m9.figshare.14318646 https://doi.org/10.1590/fst.64920 2021-11-05T12:55:41Z Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H2O2)-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss. Dataset Antarc* Antarctic Antarctic Krill Euphausia superba DataCite Metadata Store (German National Library of Science and Technology) Antarctic |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
90899 Food Sciences not elsewhere classified FOS Other engineering and technologies |
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90899 Food Sciences not elsewhere classified FOS Other engineering and technologies Shanshan WANG Deqing ZHOU Haiyan LIN Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
topic_facet |
90899 Food Sciences not elsewhere classified FOS Other engineering and technologies |
description |
Abstract Phosphorylated peptide from Antarctic krill (P-AKP) was prepared by the dry-heating method with sodium pyrophosphate in order to improve its antioxidant activity and osteogenic activity. P-AKP exhibited more competitive DPPH• and OH• scavenging activities compared to the native Antarctic krill peptide (AKP). In hydrogen peroxide (H2O2)-induced oxidative damage of MC3T3-E1 cells, both AKP and P-AKP pretreatment could dose-dependently improve superoxide dismutase (SOD) and catalase (CAT) activities through attenuating the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) production. Moreover, AKP and P-AKP prevented oxidative stress-induced down regulation of alkaline phosphatase (ALP) activity and matrix mineralization. Particularly, the promoting effects of P-AKP on the enzymatic antioxidant defense system, differentiation and mineralization was higher than that of AKP. These results suggested that phosphorylation might be a promising approach to improve the antioxidant and osteogenic activity of AKP, and P-AKP could be a beneficial agent for attenuating oxidative stress-related bone loss. |
format |
Dataset |
author |
Shanshan WANG Deqing ZHOU Haiyan LIN |
author_facet |
Shanshan WANG Deqing ZHOU Haiyan LIN |
author_sort |
Shanshan WANG |
title |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_short |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_full |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_fullStr |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_full_unstemmed |
Ameliorative effects of phosphorylated peptide from Antarctic krill (Euphausia superba) against H2O2-induced damage in MC3T3-E1 cells |
title_sort |
ameliorative effects of phosphorylated peptide from antarctic krill (euphausia superba) against h2o2-induced damage in mc3t3-e1 cells |
publisher |
SciELO journals |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.14318646 https://scielo.figshare.com/articles/dataset/Ameliorative_effects_of_phosphorylated_peptide_from_Antarctic_krill_Euphausia_superba_against_H2O2-induced_damage_in_MC3T3-E1_cells/14318646 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_relation |
https://dx.doi.org/10.1590/fst.64920 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.14318646 https://doi.org/10.1590/fst.64920 |
_version_ |
1766272192290488320 |