Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment
L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects....
Main Authors: | , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Taylor & Francis
2020
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.12948410 https://tandf.figshare.com/articles/online_resource/Glutaminase-free_L-asparaginase_production_by_i_Leucosporidium_muscorum_i_isolated_from_Antarctic_marine-sediment/12948410 |
id |
ftdatacite:10.6084/m9.figshare.12948410 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.12948410 2023-05-15T13:50:25+02:00 Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Junior, Adalberto Pessoa 2020 https://dx.doi.org/10.6084/m9.figshare.12948410 https://tandf.figshare.com/articles/online_resource/Glutaminase-free_L-asparaginase_production_by_i_Leucosporidium_muscorum_i_isolated_from_Antarctic_marine-sediment/12948410 unknown Taylor & Francis https://dx.doi.org/10.1080/10826068.2020.1815053 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Other CreativeWork Online resource article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.12948410 https://doi.org/10.1080/10826068.2020.1815053 2021-11-05T12:55:41Z L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L −1 ) and volumetric productivity (5.12 U.L −1 h −1 ). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L −1 sucrose, 15 g L −1 yeast extract and 20 g L −1 proline, which resulted in 4582.5 U L −1 and 63.64 U L −1 h −1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island DataCite Metadata Store (German National Library of Science and Technology) Antarctic King George Island |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
spellingShingle |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Junior, Adalberto Pessoa Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
topic_facet |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L −1 ) and volumetric productivity (5.12 U.L −1 h −1 ). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L −1 sucrose, 15 g L −1 yeast extract and 20 g L −1 proline, which resulted in 4582.5 U L −1 and 63.64 U L −1 h −1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. |
format |
Article in Journal/Newspaper |
author |
Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Junior, Adalberto Pessoa |
author_facet |
Freire, Rominne Karla Barros Mendonça, Carlos Miguel Nóbrega Ferraro, Rafael Bertelli Moguel, Ignacio Sánchez Tonso, Aldo Lourenço, Felipe Rebello Santos, João Henrique Picado Madalena Sette, Lara Durães Junior, Adalberto Pessoa |
author_sort |
Freire, Rominne Karla Barros |
title |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_short |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_full |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_fullStr |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_full_unstemmed |
Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment |
title_sort |
glutaminase-free l-asparaginase production by leucosporidium muscorum isolated from antarctic marine-sediment |
publisher |
Taylor & Francis |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.12948410 https://tandf.figshare.com/articles/online_resource/Glutaminase-free_L-asparaginase_production_by_i_Leucosporidium_muscorum_i_isolated_from_Antarctic_marine-sediment/12948410 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_relation |
https://dx.doi.org/10.1080/10826068.2020.1815053 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.12948410 https://doi.org/10.1080/10826068.2020.1815053 |
_version_ |
1766253474443427840 |