Glutaminase-free L-asparaginase production by Leucosporidium muscorum isolated from Antarctic marine-sediment
L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects....
Main Authors: | , , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Taylor & Francis
2020
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Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.12948410 https://tandf.figshare.com/articles/online_resource/Glutaminase-free_L-asparaginase_production_by_i_Leucosporidium_muscorum_i_isolated_from_Antarctic_marine-sediment/12948410 |
Summary: | L-asparaginase (ASNase) is an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Commercial bacterial ASNases increase patient survival, but the consequent immunological reactions remain a challenge. Yeasts ASNase is closer to human congeners and could lead to lower side effects. Among 134 yeast strains isolated from marine-sediments in King George Island, Antarctica, nine were L-asparaginase producing yeasts and glutaminase-free. Leucosporidium muscorum CRM 1648 yielded the highest ASNase activity (490.41 U.L −1 ) and volumetric productivity (5.12 U.L −1 h −1 ). Sucrose, yeast extract and proline were the best carbon and nitrogen sources to support growth and ASNase production. A full factorial design analysis pointed the optimum media condition for yeast growth and ASNase yield: 20 g L −1 sucrose, 15 g L −1 yeast extract and 20 g L −1 proline, which resulted in 4582.5 U L −1 and 63.64 U L −1 h −1 of ASNase and volumetric productivity, respectively. Analysis of temperature, pH, inoculum and addition of seawater indicated the best condition for ASNase production by this yeast: 12–15 °C, pH 5.5–6.5 and seawater >25% (v/v). Inoculum concentration seems not to interfere. This work is pioneer on the production of ASNase by cold-adapted yeasts, highlighting the potential of these microbial resources as a source of glutaminase-free L-asparaginase for commercial purposes. |
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