Nitric oxide mediates metabolic functions in the bivalve Arctica islandica under hypoxia
Short description of experiments and analyses: The formation of nitric oxide (NO) was examined in live hemocyte cells and gill filaments of the bivalve Arctica islandica at different partial pressures of oxygen (pO 2 measurements conducted with ISO-NOP electrode & confocal microscopy). To establ...
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| Format: | Dataset |
| Language: | unknown |
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figshare
2020
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| Online Access: | https://dx.doi.org/10.6084/m9.figshare.12071427.v1 https://figshare.com/articles/Nitric_oxide_mediates_metabolic_functions_in_the_bivalve_Arctica_islandica_under_hypoxia/12071427/1 |
| Summary: | Short description of experiments and analyses: The formation of nitric oxide (NO) was examined in live hemocyte cells and gill filaments of the bivalve Arctica islandica at different partial pressures of oxygen (pO 2 measurements conducted with ISO-NOP electrode & confocal microscopy). To establish whether cellular NO formation of A. islandica is catalyzed enzymatically, NO production was measured in the presence and absence of the nitric oxide synthase inhibitor (L-NAME). In a second set of experiments, the effect of chemically (by spremineNONOate) produced NO on gill respiration and cytochrome-c-oxidase activity was examined during phased reduction of pO 2 (16 kPa-2 kPa; measurements conducted with oxygen meter & spectrophotometer). Further, nitrite and nitrate contents were determined in the hemolymph of bivalves after 3.5 days of exposure to normoxic, hypoxic, and anoxic conditions (measurements conducted with microplate reader). |
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