CIL:25692, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using...

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Bibliographic Details
Main Authors: Hatano, Toshiyuki, Sluder, Greenfield
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Online Access:https://dx.doi.org/10.6075/j0w37v84
https://library.ucsd.edu/dc/object/bb4102828w
Description
Summary:Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software. ... : Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. A movie created from this series is grouped in the Cell Image Library. ...