CIL:7621, Rattus rattus, epithelial cell, intestinal epithelial cell. In Cell Image Library ...
Fixative was injected into the intestinal lumen of a rat (3% osmium tetroxide buffered at pH 7.6 with acetate- veronal). Tissue may have also been fixed in potassium permanganate. Tissue blocks were embedded in Epon. Sections were stained with lead hydroxide. Micrograph was taken at original magnifi...
| Main Authors: | , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j0w37v10 https://library.ucsd.edu/dc/object/bb78905938 |
| Summary: | Fixative was injected into the intestinal lumen of a rat (3% osmium tetroxide buffered at pH 7.6 with acetate- veronal). Tissue may have also been fixed in potassium permanganate. Tissue blocks were embedded in Epon. Sections were stained with lead hydroxide. Micrograph was taken at original magnification of 96,000 X with a Siemens Elmiskop I, operating at 60 or 80 kv, with a double condenser, and a 50 ยต objective aperture. Digitization process: conversion from a 1200 dpi tiff, 3820 x 3342 pixels. Original image created on January 5, 1962. Original resource provided as a 3.25 x 4 inch lantern slide. Research conducted at The Rockefeller Institute of Medical Research (New York, NY). ... : Preparation: osmium tetroxide fixed tissue Relation to intact cell: microtome-sectioned tissue Item type: film Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain Visualization methods: lead salt Processing history: digitized lantern slide Data qualification: Processed;spatialmeasurements;intensitiesquantitation ... |
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