CIL:25305, Drosophila melanogaster. In Cell Image Library ...
Confocal fluorescent time-lapse imaging of bipolar spindle collapse induced by 1 uM taxol in a mitotic Drosophila S2 cell expressing GFP-tubulin. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens...
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Format: | Dataset |
Language: | unknown |
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UC San Diego Library Digital Collections
2021
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Online Access: | https://dx.doi.org/10.6075/j0v40t0h https://library.ucsd.edu/dc/object/bb89145895 |
Summary: | Confocal fluorescent time-lapse imaging of bipolar spindle collapse induced by 1 uM taxol in a mitotic Drosophila S2 cell expressing GFP-tubulin. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 6 in Maresca and Salmon, 2009 J Cell Biol 184:373-281. ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: differences in adsorption or binding of stain Visualization methods: Green fluorescent proteins from Aequorea Processing history: unprocessed raw data ... |
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