CIL:39977, Phaeocystis antarctica, algae. In Cell Image Library ...
Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment Relation to intact cell: microtome-sectioned tissue Imaging mode: illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: stain with broad specifi...
| Main Authors: | , , , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j0rv0nth https://library.ucsd.edu/dc/object/bb1270661j |
| Summary: | Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment Relation to intact cell: microtome-sectioned tissue Imaging mode: illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: stain with broad specificity Visualization methods: lead salt; uranyl salt; cationic colloidal gold Processing history: recorded image Data qualification: Processed ... : Culture conditions. Cultures of colonial P. antarctica (CCMP 1374) were grown semi-continuously for 5-8 generations in f/2 medium (Guillard and Ryther 1962) under continuous blue light at 4°C at irradiances of 14 and 259 µmol quanta m-2 s-1. Specific growth rate. Specific growth rate was estimated by a linear regression of loge transformed daily determinations of in vivo fluorescence intensity (n=2) measured with a Turner Model 10 fluorometer. Sample preparation for electron microscopy. P. antarctica colonies were fixed on ice with a 2% glutaraldehyde and 1.3% osmium tetroxide solution for 30 minutes and rinsed in distilled water. Cells were dehydrated through a series of ethanol: water washes (25:75, 50:50, 75:25, 95:5), three 100% ethanol washes and finally through three washes of 100% acetone. Cells were pelleted and fixed in an Epon resin. The fixation process lends itself to a breakup of the colonial matrix and we were able to examine P. antarctica individual colonial cells using electron tomography. ... |
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