CIL:35201, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera usin...

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Bibliographic Details
Main Authors: Uetake, Yumi, Sluder, Greenfield
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Online Access:https://dx.doi.org/10.6075/j0rb73hb
https://library.ucsd.edu/dc/object/bb53997811
Description
Summary:Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software. ... : Movie showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1 using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671). The time series used to prepare the movie is grouped in the Cell Image Library. ...