CIL:7239, Homo sapiens, epithelial cell. In Cell Image Library ...
Preparation: methanol fixed tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: primary antibody plus label...
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Online Access: | https://dx.doi.org/10.6075/j0r210f5 https://library.ucsd.edu/dc/object/bb8880848q |
| Summary: | Preparation: methanol fixed tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); Alexa Fluor 594; labeled primary antibody Processing history: unprocessed raw data ... : HeLa cells were accumulated in monopolar mitosis using a 12 hr treatment of the kinesin-5 inhibitor S-trityl-l-cysteine and forced into cytokinesis by adding the potent Cdk1 inhibitor purvalanol A for 15 min. The microtubules (green) were not growing and shrinking. Aurora B (red), which functions in attaching microtubules to the centromere, localizes at the gap region between the cortex and the microtubule plus ends. Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against Auruoa B and FITC-DM1a antibody against microtubules. Secondary antibody was Alexa 594 for Aurora B. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an Orca ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52. ... |
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