CIL:11839, Potorous tridactylus, epithelial cell. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocess...

Full description

Bibliographic Details
Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
microtubule
microtubule polymerization
epithelial cell
Pt K1 NBL-3
activated Rac
Potorous tridactylus
Orca
Online Access:https://dx.doi.org/10.6075/j0np235g
https://library.ucsd.edu/dc/object/bb4955416g
Description
Summary:Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubule fragments exhibiting net treadmilling in a Rac1(Q61L)-expressing PtK1 cell. Microtubule plus ends undergo dynamic instability while their minus ends depolymerize, resulting in net treadmilling of the microtubule lattice. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). For still images, see Fig. 2 D of associated paper (J Cell Biol, 161:845-851, 2003) ...

Similar Items