CIL:25359, Canis lupus familiaris, epithelial cell. In Cell Image Library ...

• Main Authors: Pentecost, M, Otto, G., Theriot, J.A., Amieva, M.R.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: cell extrusion; epithelial cell; MDCK; cell; Canis lupus familiaris; Canis lupus
• Online Access: https://dx.doi.org/10.6075/j0ng4pdb; https://library.ucsd.edu/dc/object/bb12694694

Preparation: living tissue Relation to intact cell: monolayer Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27. ...