CIL:25359, Canis lupus familiaris, epithelial cell. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: monolayer Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: vis...

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Bibliographic Details
Main Authors: Pentecost, M, Otto, G., Theriot, J.A., Amieva, M.R.
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Online Access:https://dx.doi.org/10.6075/j0ng4pdb
https://library.ucsd.edu/dc/object/bb12694694
Description
Summary:Preparation: living tissue Relation to intact cell: monolayer Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27. ...