CIL:37933, Caenorhabditis elegans, early embryonic cell. In Cell Image Library ...

C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objec...

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Bibliographic Details
Main Authors: Goehring, Nathan W., Hoege, Carsten, Grill, Stephan W., Hyman, Anthony A.
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
cell cortex
early embryonic cell
Caenorhabditis elegans
embryo development ending in birth or egg hatching
anterior/posterior axis specification, embryo
establishment or maintenance of polarity of embryonic epithelium
Orca
Online Access:https://dx.doi.org/10.6075/j0mg7n62
https://library.ucsd.edu/dc/object/bb9392538p
Description
Summary:C. elegans embryo expressing mCherry::PAR-2 (green) and GFP::PAR-6 (red) was treated with cytochalasin-D. Embryo was made permeable by placing L4 larvae on F08F8.2(RNAi) plates for 20-24h. Images were captured by wide-field fluorescence microscopy (Zeiss Axioplan II, 63x/1.4 Oil PlanApochromat objective, Hamamatsu Orca-ER camera) at 30 second intervals. Time-lapse images correspond to Video1 (at 8 frames/second) and Fig 4C in J Cell Biol. 2011. 193(3): 583-594. ... : Anterior and posterior regions of a C. elegans embryo are determined by PAR protein distribution. PAR-2, shown in green marks the posterior region of the embryo and PAR-6, shown in red, marks the anterior region. DNA is in blue. The embryo was treated with the drug cytochalasin D (disrupts the actin cytoskeleton) at frame 19. In the presence of the drug, PAR domains remain stable until anaphase when PAR-2 is lost to invaginations. ...

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