CIL:30560, Drosophila melanogaster, early embryonic cell. In Cell Image Library ...
A confocal Z-stack from a transgenic Drosophila embryo expressing the kinesin-14 motor Ncd-GFP during prometaphase phase 1. This image stack is original data contributing to Fig. 1 "Prometaphase spindle dynamics in Drosophila embryos: steady-state and elongation phase" from Civelekoglu-Sch...
| Main Authors: | , , , , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j0m61hvw https://library.ucsd.edu/dc/object/bb1337794q |
| Summary: | A confocal Z-stack from a transgenic Drosophila embryo expressing the kinesin-14 motor Ncd-GFP during prometaphase phase 1. This image stack is original data contributing to Fig. 1 "Prometaphase spindle dynamics in Drosophila embryos: steady-state and elongation phase" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68. ... : Embryos expressing GFP-Ncd were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). For these experiments, z-stacks were acquired with an Orca II CCD camera (Hamamatsu Photonics) at intervals of 0.1-0.2 µm, using exposure times of 300-600ms. For further information see J. Cell Biol. 188(1):49-68. ... |
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