CIL:13161, Mus musculus, fibroblast. In Cell Image Library ...

Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and...

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Bibliographic Details
Main Authors: Doyle, Andrew D., Wang, Francis W., Matsumoto, Kazue, Yamada, Kenneth M.
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Mus musculus
cell migration
NIH/3T3
cell
fibroblast
substrate-dependent cell migration, cell attachment to substrate
Orca
Online Access:https://dx.doi.org/10.6075/j0m0445f
https://library.ucsd.edu/dc/object/bb10304991
Description
Summary:Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 2 min and shown at 15 frames per second. ... : Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: phase contrast microscopy Parameter imaged: elastic scattering of photons Source of contrast: differences in amount of elastic light scattering Visualization methods: visualization of contiguous regions Processing history: custom-made smoothing, sharpening, and convolution filters using MetaMorph software Data qualification: Processed ...

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