CIL:40376, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library ...

Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite...

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Bibliographic Details
Main Authors: Ferenz, Nick, Wadsworth, Patricia
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Sus scrofa domestica
mitotic metaphase
microtubule cytoskeleton organization
LLC-PK1
kidney epithelial cell
microtubule cytoskeleton
mitosis
Orca
Online Access:https://dx.doi.org/10.6075/j0jq10bh
https://library.ucsd.edu/dc/object/bb3625165j
Description
Summary:Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp. See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: EGFP Data qualification: Processed ...

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