CIL:7620, Rattus rattus, epithelial cell, intestinal epithelial cell. In Cell Image Library ...
Fixative was injected into the intestinal lumen of a rat (3% osmium tetroxide buffered at pH 7.6 with acetate- veronal). Tissue may have also been fixed in potassium permanganate. Tissue blocks were embedded in Epon. Sections were stained with lead hydroxide. Micrograph was taken at original magnifi...
| Main Authors: | , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j0gh9gnp https://library.ucsd.edu/dc/object/bb4409333g |
| Summary: | Fixative was injected into the intestinal lumen of a rat (3% osmium tetroxide buffered at pH 7.6 with acetate- veronal). Tissue may have also been fixed in potassium permanganate. Tissue blocks were embedded in Epon. Sections were stained with lead hydroxide. Micrograph was taken at original magnification of 96,000 X with a Siemens Elmiskop I, operating at 60 or 80 kv, with a double condenser, and a 50 ยต objective aperture. Digitization process: conversion from a 1200 dpi tiff, 3820 x 3342 pixels. Original image created on January 5, 1962. Original resource provided as a 3.25 x 4 inch lantern slide. Research conducted at The Rockefeller Institute of Medical Research (New York, NY). ... : Junctional complex between two cells in the epithelium of the rat intestinal mucosa. The tight junction (also known as zonula occludens) is the structure located nearest to the lumen. The elimination of the intercellular gap (approx 90 angstroms) is clearly visible, but the fusion line of the two apposed membranes cannot be clearly distinguished at this magnification. The adherens junction (also known as zonula adherens) is the next structure. A relatively wide intercellular space (approx 200 angstroms) is maintained throughout this junction. Extensive actin filament networks are seen along both sides of the junction which are continuous with the terminal web into which the filamentous rootlets of the mircovilli penetrate. Dense material can be seen along parts of both sides of the junction. The limits of a desmosome are marked by the edges of the dense plaques. Desmosomes are characterized by a wide intercellular space (approx 240 angstroms) bisected by an intermediate line. Bundles of intermediate ... |
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