CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. In Cell Image Library ...
Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: TIRF Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data ... : Visualiz...
| Main Authors: | , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Online Access: | https://dx.doi.org/10.6075/j0dn4454 https://library.ucsd.edu/dc/object/bb3454268k |
| Summary: | Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: TIRF Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: EGFP Processing history: unprocessed raw data ... : Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm ... |
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