CIL:34848, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Vi...

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Bibliographic Details
Main Authors: Hatano, Toshiyuki, Sluder, Greenfield
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
cell proliferation
mitosis
retinal pigment epithelium
Homo sapiens
hTERT-RPE1
Orca
Online Access:https://dx.doi.org/10.6075/j0cr5s70
https://library.ucsd.edu/dc/object/bb64578071
Description
Summary:Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 objective lens. images were captured at 30 sec intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software. ...

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