CIL:11841, Potorous tridactylus, epithelial cell. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocess...

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Bibliographic Details
Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
activated Rac
PAK inhibition
Pt K1 NBL-3
microtubule
epithelial cell
microtubule polymerization
Potorous tridactylus
Orca
Online Access:https://dx.doi.org/10.6075/j09p30c6
https://library.ucsd.edu/dc/object/bb74469070
Description
Summary:Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 4, B and D of associated paper (J Cell Biol, 161:845-851, 2003). ...

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