CIL: 50722, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library ...

• Main Authors: Ivanchenko, Maryna V., Cicconet, Marcelo, Jandal, Hoor Al, Wu, Xudong, Corey, David P., Indzhykulian, Artur A.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: Native auditory Hair Cells at postnatal day 4; Hair cell stereocilia; mitochondria; Mus musculus; Cuticular plate; Polycystic Kidney and Hepatic Disease 1-Like 1 PKHD1L1 labeling gold beads; Inner ear outer hair cell; Helios; Orca
• Online Access: https://dx.doi.org/10.6075/j05b01v2; https://library.ucsd.edu/dc/object/bb18525947

Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 6.4_Cell_6_GoldParticle_Segmentation is a multipage TIFF file illustrating the gold bead segmentation results. Same as ‘GoldParticles.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview. File contains 324 images, as all the planes that contained no segmentation data were removed. This file is a part of Dataset 6, comprised of files related to the Cell #6 of current study. The numbering order of the files reflects the order of operations performed during the analysis. The raw imaging data related to this cell was deposited within Dataset 1 (CIL:50680). ...