CIL:13607, Homo sapiens, fibroblast. In Cell Image Library ...
Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on DMI6000B Leica microscope using a 100X 1.4 NA object...
Main Authors: | , , , , , |
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Format: | Dataset |
Language: | unknown |
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UC San Diego Library Digital Collections
2021
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Subjects: | |
Online Access: | https://dx.doi.org/10.6075/j04j0cth https://library.ucsd.edu/dc/object/bb0006618k |
Summary: | Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on DMI6000B Leica microscope using a 100X 1.4 NA objective lens and subsequently processed by deconvolution. ... : Behavior of a patient-derived human fibroblast line (PD-NC4) containing a mutated chromosome 4 in which the normal centromere is epigenetically silenced and a neo-centromere has formed 25 Mbp down the q arm at a site that lacks centromere repeats. Shown is a z-series of a metaphse spread stained with anti-centromere antibody (ACA, green) which labels both the epigenetically silenced centromere and the neocentromere of chromosome 4 which appears in the lower left quadrant in the z-slice displayed. Also stained for DNA with DAPI (blue). See Fig 1A in Bassett et al. 2010. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. J Cell Biol 190:177-185. ... |
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