CIL:11835, Potorous tridactylus, epithelial cell. In Cell Image Library ...

• Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: microtubule; microtubule polymerization; Potorous tridactylus; epithelial cell; Pt K1 NBL-3; Orca
• Online Access: https://dx.doi.org/10.6075/j00p0xst; https://library.ucsd.edu/dc/object/bb1235245d

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tubulin and observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 1 of associated manuscript (J Cell Biol, 161:845-851, 2003). ...