CIL:11835, Potorous tridactylus, epithelial cell. In Cell Image Library ...
Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocess...
Main Authors: | , , |
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Format: | Dataset |
Language: | unknown |
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UC San Diego Library Digital Collections
2021
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Subjects: | |
Online Access: | https://dx.doi.org/10.6075/j00p0xst https://library.ucsd.edu/dc/object/bb1235245d |
Summary: | Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tubulin and observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 1 of associated manuscript (J Cell Biol, 161:845-851, 2003). ... |
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