CIL:13608, Homo sapiens, fibroblast. In Cell Image Library ...
Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on DMI6000B Leica microscope using a 100X 1.4 NA object...
| Main Authors: | , , , , , |
|---|---|
| Format: | Dataset |
| Language: | unknown |
| Published: |
UC San Diego Library Digital Collections
2021
|
| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j00000t6 https://library.ucsd.edu/dc/object/bb60817599 |
| Summary: | Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on DMI6000B Leica microscope using a 100X 1.4 NA objective lens and subsequently processed by deconvolution. ... : Behavior of a patient-derived human fibroblast line (PD-NC4) containing a mutated chromosome 4 in which the normal centromere is epigenetically silenced and a neo-centromere has formed 25 Mbp down the q arm at a site that lacks centromere repeats. Shown is a z-series of the PD-NC4 chromosome from a metaphase spread stained for CENP-B (red), centromere (ACA, green) and DNA (DAPI blue). See Fig 1D in Bassett et al. 2010. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. J Cell Biol 190:177-185. ... |
|---|