Photosynthetic pigments of water column samples and analyzed with High Performance Liquid Chromatography (HPLC), collected aboard Palmer LTER annual cruises off the coast of the Western Antarctica Peninsula, 1991 - 2016.
Phytoplankton pigment sampling was led by Prezelin from 1991-1994, and then by Vernet from 1995-2008. Schofield is the third, and current lead, beginning in 2009. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have...
| Main Authors: | , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Environmental Data Initiative
2018
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6073/pasta/4d583713667a0f52b9d2937a26d0d82e https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-pal.42.8 |
| Summary: | Phytoplankton pigment sampling was led by Prezelin from 1991-1994, and then by Vernet from 1995-2008. Schofield is the third, and current lead, beginning in 2009. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Phytoplankton have a suite of accessory pigments in addition to Chlorophyll a, including other Chlorophyll’s (e.g. Chlorophyll b), Xanthophylls, and Carotenes. These accessory pigments can be used as chemotaxonomic markers to assess the composition and distribution of the phytoplankton community. For example, Fucoxanthin is a marker pigment of Diatoms, whereas Alloxanthin is a marker pigment of Cryptophytes. Accessory pigments also assist in photoacclimation and photoprotective processes. Water samples are collected throughout the water column along the Western Antarctic Peninsula at regular LTER grid stations where CTD casts are preformed and in surface waters at underway stations, where CTD casts are not done, using the ship’s flow-through seawater system. Water samples are filtered onto GF/F filters, and filters kept frozen at -80C until analysis. HPLC analysis is completed following Wright et al (1991). Following the guidelines set by NASA SeaHARRE, we use an internal standard and replicate injects on the HPLC to track recovery and replicability of the pigment extraction methods and the HPLC. Data is unavailable for the LMG10-01 cruise due to instrumentation problems and for the LMG12-01 cruise due to a freezer failure which resulted in the loss of samples. |
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