Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of bacterial 16S rRNA genes. Soil samples taken from Long-term Ecological Research (LTER) greenhouse treated and control plots at Toolik Lake, AK; 5 sampling dates.

In the summer of 2006 and 2007, we collected soils treated with warming by greenhouses since 1989, which are maintained as part of the Arctic Long-term Ecological Research (LTER) site at Toolik Lake, AK (68o38'N, 149o34'W, elevation 760m). Total nucleic acids were extracted from soil sampl...

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Bibliographic Details
Main Authors: Simard, Suzanne, Deslippe, Julie R., E. Gwenn Farrell, Gaius Shaver, Hartmann, Martin, Mohn, William
Format: Dataset
Language:English
Published: Canadian Cryospheric Information Network 2016
Subjects:
Bacteria
DNA
Genetic analysis
Soil microbiology
Soil temperature
Temperature
Tundra
International Polar Year-Carbon-flux and plant-microbial community dynamics in Low-Arctic tundra
Arctic
International Polar Year
Online Access:https://dx.doi.org/10.5443/704
https://www.polardata.ca/pdcsearch/?doi_id=704
Description
Summary:In the summer of 2006 and 2007, we collected soils treated with warming by greenhouses since 1989, which are maintained as part of the Arctic Long-term Ecological Research (LTER) site at Toolik Lake, AK (68o38'N, 149o34'W, elevation 760m). Total nucleic acids were extracted from soil samples allowing for the simultaneous assessment of the total and actively growing components of the soil microbial community. We used Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis of 16S rRNA genes to profile the bacterial community. : Purpose: Assessment of long-term warming on structure and function of soil microbial communities. : Summary: Not Applicable

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