Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 2.0 ...

• Main Author: Newsham, Kevin K.
• Format: Dataset
• Language: English
• Published: NERC EDS UK Polar Data Centre 2023
• Subjects: "EARTH SCIENCE","BIOLOGICAL CLASSIFICATION","FUNGI"; Antarctic heatwaves; beta-glucosidase; cellulase; fluctuating temperature; growth rate; psychrotrophy; soil fungi; Alexander Island; Antarctic; Lewis Pass; Mars Oasis; Signy Island; The Backslope; Antarc*; Antarctica; British Antarctic Survey
• Online Access: https://dx.doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3; https://data.bas.ac.uk/full-record.php?id=GB/NERC/BAS/PDC/01807

Data on the growth rates and specific cellulase activities of 10 isolates of the fungus Pseudogymnoascus roseus from sub- and maritime Antarctica, exposed in vitro to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius and their corresponding mean constant temperatures of 9.0 degrees Celsius, 10.7, 12.0 or 13.1 degrees Celsius, respectively, and constant temperatures of 2 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius. Funding: UK Natural Environment Research Council (core funding to the British Antarctic Survey). ... : A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium. Three experiments were conducted. In the first, each isolate was inoculated onto soil extract medium in eight Petri dishes, which were incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony fronts were then marked on dish reverses with a fine blade at x 10-15 magnification (accuracy c. 100 micrometers) and four dishes inoculated with each isolate were transferred to cabinets fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius. The other four dishes inoculated with each isolate were transferred back to the ...