Deschampsia antarctica É.Desv. ...
2.1. Analysis of CHS gene from D. antarctica DaCHS full-length cDNA was obtained using the partial sequence of an EST as the template, designing primers for 5′- and 3′-RACE-PCR reactions. A sequence of 1741 bp with a poly (A) tail, containing 143 bp and 407 bp of 5′- and 3′ -UTR sequences respective...
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ftdatacite:10.5281/zenodo.8301817 2023-10-01T03:52:07+02:00 Deschampsia antarctica É.Desv. ... Cuadra, Pedro Guajardo, Joselin Carrasco-Orellana, Cristian Stappung, Yazmina Fajardo, Víctor Herrera, Raúl 2020 https://dx.doi.org/10.5281/zenodo.8301817 https://zenodo.org/record/8301817 unknown Zenodo http://zenodo.org/record/8293717 http://publication.plazi.org/id/FFEE6314FFFFFB66600CFFDCFFA0C746 https://sibils.text-analytics.ch/search/collections/plazi/03D71B6CFFFEFB63633EFD11FAF6C369 https://zenodo.org/communities/biosyslit https://dx.doi.org/10.1016/j.phytochem.2019.112179 http://zenodo.org/record/8293717 http://publication.plazi.org/id/FFEE6314FFFFFB66600CFFDCFFA0C746 https://sibils.text-analytics.ch/search/collections/plazi/03D71B6CFFFEFB63633EFD11FAF6C369 https://dx.doi.org/10.5281/zenodo.8293719 https://dx.doi.org/10.5281/zenodo.8293721 https://dx.doi.org/10.5281/zenodo.8293723 https://dx.doi.org/10.5281/zenodo.8293725 https://dx.doi.org/10.5281/zenodo.8293727 https://dx.doi.org/10.5281/zenodo.8293729 https://dx.doi.org/10.5281/zenodo.8301816 https://zenodo.org/communities/biosyslit Open Access Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 info:eu-repo/semantics/openAccess Biodiversity Taxonomy Plantae Tracheophyta Liliopsida Poales Poaceae Deschampsia Deschampsia antarctica article-journal Taxonomic treatment ScholarlyArticle Text 2020 ftdatacite https://doi.org/10.5281/zenodo.830181710.1016/j.phytochem.2019.11217910.5281/zenodo.829371910.5281/zenodo.829372110.5281/zenodo.829372310.5281/zenodo.829372510.5281/zenodo.829372710.5281/zenodo.829372910.5281/zenodo.8301816 2023-09-04T15:21:51Z 2.1. Analysis of CHS gene from D. antarctica DaCHS full-length cDNA was obtained using the partial sequence of an EST as the template, designing primers for 5′- and 3′-RACE-PCR reactions. A sequence of 1741 bp with a poly (A) tail, containing 143 bp and 407 bp of 5′- and 3′ -UTR sequences respectively, was obtained as well as an ORF of 1191 bp with a deduced polypeptide sequence of 397 amino acids. This sequence was deposited in GenBank (accession number MG766286). Analysis of the predicted DaCHS protein demonstrated the typical conserved structural features among CHSs. The mature protein has an estimated molecular weight of 43.53 kDa (pI 6.44). Important residues for the active site motif can be observed in DaCHS (C167, F218, H306 and N339). Moreover, the active site motif is formed by a variety of residues typical of the chalcone synthase family, which are also observed in the Deschampsia protein sequence. It has two domains: the N-terminal domain ranges from amino acid 8 to 231 and the C-terminal domain ... : Published as part of Cuadra, Pedro, Guajardo, Joselin, Carrasco-Orellana, Cristian, Stappung, Yazmina, Fajardo, Víctor & Herrera, Raúl, 2020, Differential expression after UV-B radiation and characterization of chalcone synthase from the Patagonian hairgrass Deschampsia antarctica, pp. 1-10 in Phytochemistry (112179) (112179) 169 on pages 2-6, DOI: 10.1016/j.phytochem.2019.112179, http://zenodo.org/record/8293717 ... Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) Raúl ENVELOPE(-65.700,-65.700,-68.167,-68.167) Carrasco ENVELOPE(-63.367,-63.367,-64.800,-64.800) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biodiversity Taxonomy Plantae Tracheophyta Liliopsida Poales Poaceae Deschampsia Deschampsia antarctica |
spellingShingle |
Biodiversity Taxonomy Plantae Tracheophyta Liliopsida Poales Poaceae Deschampsia Deschampsia antarctica Cuadra, Pedro Guajardo, Joselin Carrasco-Orellana, Cristian Stappung, Yazmina Fajardo, Víctor Herrera, Raúl Deschampsia antarctica É.Desv. ... |
topic_facet |
Biodiversity Taxonomy Plantae Tracheophyta Liliopsida Poales Poaceae Deschampsia Deschampsia antarctica |
description |
2.1. Analysis of CHS gene from D. antarctica DaCHS full-length cDNA was obtained using the partial sequence of an EST as the template, designing primers for 5′- and 3′-RACE-PCR reactions. A sequence of 1741 bp with a poly (A) tail, containing 143 bp and 407 bp of 5′- and 3′ -UTR sequences respectively, was obtained as well as an ORF of 1191 bp with a deduced polypeptide sequence of 397 amino acids. This sequence was deposited in GenBank (accession number MG766286). Analysis of the predicted DaCHS protein demonstrated the typical conserved structural features among CHSs. The mature protein has an estimated molecular weight of 43.53 kDa (pI 6.44). Important residues for the active site motif can be observed in DaCHS (C167, F218, H306 and N339). Moreover, the active site motif is formed by a variety of residues typical of the chalcone synthase family, which are also observed in the Deschampsia protein sequence. It has two domains: the N-terminal domain ranges from amino acid 8 to 231 and the C-terminal domain ... : Published as part of Cuadra, Pedro, Guajardo, Joselin, Carrasco-Orellana, Cristian, Stappung, Yazmina, Fajardo, Víctor & Herrera, Raúl, 2020, Differential expression after UV-B radiation and characterization of chalcone synthase from the Patagonian hairgrass Deschampsia antarctica, pp. 1-10 in Phytochemistry (112179) (112179) 169 on pages 2-6, DOI: 10.1016/j.phytochem.2019.112179, http://zenodo.org/record/8293717 ... |
format |
Text |
author |
Cuadra, Pedro Guajardo, Joselin Carrasco-Orellana, Cristian Stappung, Yazmina Fajardo, Víctor Herrera, Raúl |
author_facet |
Cuadra, Pedro Guajardo, Joselin Carrasco-Orellana, Cristian Stappung, Yazmina Fajardo, Víctor Herrera, Raúl |
author_sort |
Cuadra, Pedro |
title |
Deschampsia antarctica É.Desv. ... |
title_short |
Deschampsia antarctica É.Desv. ... |
title_full |
Deschampsia antarctica É.Desv. ... |
title_fullStr |
Deschampsia antarctica É.Desv. ... |
title_full_unstemmed |
Deschampsia antarctica É.Desv. ... |
title_sort |
deschampsia antarctica é.desv. ... |
publisher |
Zenodo |
publishDate |
2020 |
url |
https://dx.doi.org/10.5281/zenodo.8301817 https://zenodo.org/record/8301817 |
long_lat |
ENVELOPE(-65.700,-65.700,-68.167,-68.167) ENVELOPE(-63.367,-63.367,-64.800,-64.800) |
geographic |
Raúl Carrasco |
geographic_facet |
Raúl Carrasco |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://zenodo.org/record/8293717 http://publication.plazi.org/id/FFEE6314FFFFFB66600CFFDCFFA0C746 https://sibils.text-analytics.ch/search/collections/plazi/03D71B6CFFFEFB63633EFD11FAF6C369 https://zenodo.org/communities/biosyslit https://dx.doi.org/10.1016/j.phytochem.2019.112179 http://zenodo.org/record/8293717 http://publication.plazi.org/id/FFEE6314FFFFFB66600CFFDCFFA0C746 https://sibils.text-analytics.ch/search/collections/plazi/03D71B6CFFFEFB63633EFD11FAF6C369 https://dx.doi.org/10.5281/zenodo.8293719 https://dx.doi.org/10.5281/zenodo.8293721 https://dx.doi.org/10.5281/zenodo.8293723 https://dx.doi.org/10.5281/zenodo.8293725 https://dx.doi.org/10.5281/zenodo.8293727 https://dx.doi.org/10.5281/zenodo.8293729 https://dx.doi.org/10.5281/zenodo.8301816 https://zenodo.org/communities/biosyslit |
op_rights |
Open Access Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.5281/zenodo.830181710.1016/j.phytochem.2019.11217910.5281/zenodo.829371910.5281/zenodo.829372110.5281/zenodo.829372310.5281/zenodo.829372510.5281/zenodo.829372710.5281/zenodo.829372910.5281/zenodo.8301816 |
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