Deschampsia antarctica É.Desv. ...
2.1. Analysis of CHS gene from D. antarctica DaCHS full-length cDNA was obtained using the partial sequence of an EST as the template, designing primers for 5′- and 3′-RACE-PCR reactions. A sequence of 1741 bp with a poly (A) tail, containing 143 bp and 407 bp of 5′- and 3′ -UTR sequences respective...
| Main Authors: | , , , , , |
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| Format: | Text |
| Language: | unknown |
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Zenodo
2020
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| Online Access: | https://dx.doi.org/10.5281/zenodo.8301817 https://zenodo.org/record/8301817 |
| Summary: | 2.1. Analysis of CHS gene from D. antarctica DaCHS full-length cDNA was obtained using the partial sequence of an EST as the template, designing primers for 5′- and 3′-RACE-PCR reactions. A sequence of 1741 bp with a poly (A) tail, containing 143 bp and 407 bp of 5′- and 3′ -UTR sequences respectively, was obtained as well as an ORF of 1191 bp with a deduced polypeptide sequence of 397 amino acids. This sequence was deposited in GenBank (accession number MG766286). Analysis of the predicted DaCHS protein demonstrated the typical conserved structural features among CHSs. The mature protein has an estimated molecular weight of 43.53 kDa (pI 6.44). Important residues for the active site motif can be observed in DaCHS (C167, F218, H306 and N339). Moreover, the active site motif is formed by a variety of residues typical of the chalcone synthase family, which are also observed in the Deschampsia protein sequence. It has two domains: the N-terminal domain ranges from amino acid 8 to 231 and the C-terminal domain ... : Published as part of Cuadra, Pedro, Guajardo, Joselin, Carrasco-Orellana, Cristian, Stappung, Yazmina, Fajardo, Víctor & Herrera, Raúl, 2020, Differential expression after UV-B radiation and characterization of chalcone synthase from the Patagonian hairgrass Deschampsia antarctica, pp. 1-10 in Phytochemistry (112179) (112179) 169 on pages 2-6, DOI: 10.1016/j.phytochem.2019.112179, http://zenodo.org/record/8293717 ... |
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