VoltView 1.0 to analyze all-optical Voltron imaging with Example Data ...
VoltVew 1.0 is an analysis package written in MATLAB by the Fuzik Laboratory at Karolinska Institute."VoltView_README.pdf" describes the installation of VoltView 1.0 and its dependencies (MATLAB 2021, bfmatlab, Anaconda3, Cellpose 1.0 in Python 3.8). We included example files: The ".C...
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| Format: | Software |
| Language: | English |
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Zenodo
2023
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| Online Access: | https://dx.doi.org/10.5281/zenodo.8030175 https://zenodo.org/record/8030175 |
| Summary: | VoltVew 1.0 is an analysis package written in MATLAB by the Fuzik Laboratory at Karolinska Institute."VoltView_README.pdf" describes the installation of VoltView 1.0 and its dependencies (MATLAB 2021, bfmatlab, Anaconda3, Cellpose 1.0 in Python 3.8). We included example files: The ".CXD" video files were generated with a Hamamatsu Orca Fusion-BT camera. Imaging frame rate was 600 Hz. Imaging magnification was 10X. Each video file contains an all-optical voltage imaging experiment looking at periaqueductal gray (PAG) neurons during optogenetic stimulation of ventromedial hypothalamic terminals in the PAG. Each experiment has 7 sweeps, each sweep has a baseline (0.5 s), a 20 Hz optogenetic terminal stimulation with 2 ms light pulses (1s), and a follow up (~0.9 s). Other details and parameters are described in the manuscript "Voltage-Seq: all-optical postsynaptic connectome-guided single-cell transcriptomics" (https://www.biorxiv.org/content/10.1101/2022.11.09.515875v2) ... |
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