Pseudomonas stutzeri MLA9, una cepa marina con alto potencial para degradar pireno

• Main Authors: Araujo-Palomares, Cynthia Lizzeth, Ramos- Mendoza, Ileana Sarahí, Silva-Jiménez, Hortencia
• Format: Text
• Language: Spanish
• Published: Zenodo 2019
• Subjects: Bacterias hidrocarbonoclásticas; PAH; Pseudomonas stutzeri; pireno; Hydrocarbonoclastic bacteria, Pseudomonas stutzerí, PAH, pyrene; Indian; Aitken; Myers; Ruta; Barra; Ferraz; Mitra; Romero; O'Connor; Toth; Eklund; Artide
• Online Access: https://dx.doi.org/10.5281/zenodo.5090448; https://zenodo.org/record/5090448

RESUMEN Una alternativa para remover los hidrocarburos aromáticos policíclicos (HAP) del ambiente es usando bacterias hidrocarbonoclastas. El objetivo de este trabajo fue comenzar con la caracterización de la cepa bacteriana marina Pseudomonas stutzeri MLA9 con potencial para degradar el pireno. La cepa bacteriana previamente fue identificada por el sistema MALDI-Biotyper y confirmado en este estudio por la secuenciación del gen ribosomal 16S como Pseudomonas stutzeri. La cepa bacteriana puede crecer en medio mínimo suplementario con pireno, fenantreno o naftaleno como su única fuente de carbono y energía, siendo el pireno su mejor sustrato. Análisis moleculares han permitido la detección de genes que codifican para dioxigenasas de hidroxilación y de escisión, enzimas que participan en la ruta de degradación de HAP. Además, P. stutzeri MLA9 es una cepa productora de biosurfactantes y formadora de biopelículas, mecanismos que pueden facilitar el proceso de degradación. Lo anterior apunta a P. stutzeri MLA9 como una excelente candidata para continuar los estudios de degradación de HAP y su posible aplicación en procesos de biorremediación. ABSTRACT An alternative to remove polycyclic aromatic hydrocarbons (PAH) of the environment is using hydrocarbonoclastic bacteria. The aim of this work was to begin with the characterization of the marine bacterial strain MLA9 with potential to degrade pyrene. The isolate was previously identifying used MALDI-Biotyper and, ¡n this study, its identity was confirmed by 16S ribosomal gene sequencing as Pseudomonas stutzerí. The bacterial strain can grow in minimal médium supplemented with pyrene, phenanthrene or naphthalene as solé carbón and energy source, being pyrene the best substrate. Molecular analysis has allowed hydroxylating and excisión dioxygenases genes detection, enzymes that participating in PAH degrading pathway. 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