mRNA expression data of genes related to mitochondrial quality control in hepatopancreas of the two marine bivalves, Mytilus edulis and Crassostrea gigas, during short-term hypoxia/reoxygenation stress

• Main Authors: Steffen, Jennifer Barbara Maria, Falfushynska, Halina, Piontkivska, Helen, Sokolova, Inna M.
• Format: Dataset
• Language: unknown
• Published: Zenodo 2020
• Subjects: Mitochondria; Fission; Fusion; Mitophagy; mRNA expression; Mollusca; Pacific; Sokolova; Crassostrea gigas
• Online Access: https://dx.doi.org/10.5281/zenodo.4266408; https://zenodo.org/record/4266408

Coastal environments commonly experience strong oxygen fluctuations. Resulting hypoxia/reoxygenation stress can negatively affect mitochondrial functions, since oxygen deficiency impairs ATP generation, whereas a surge of oxygen causes mitochondrial damage by oxidative stress mechanisms. Marine intertidal bivalves are adapted to fluctuating oxygen conditions, yet the underlying molecular mechanisms that sustain mitochondrial integrity and function during oxygen fluctuations are not yet well understood. We used targeted mRNA expression analysis to determine the potential involvement of the mitochondrial quality control mechanisms in responses to short-term hypoxia (24 h at <0.01% O 2 ) and subsequent reoxygenation (1.5 h at 21% O 2 ) in two hypoxia-tolerant marine bivalves, the Pacific oysters Crassostrea gigas and the blue mussels Mytilus edulis . To test these hypotheses, We focused on the transcript levels of the following marker genes: for mitochondrial fission and fusion - mfn 2 (encoding mitofusin 2), opa 1 (mitochondrial dynamin-like 120kDa protein), dnm 1 l (dynamin-1-like protein), mff (mitochondrial fission factor), fis 1 (mitochondrial fission protein 1); for protein and DNA quality control - tsfm (encoding mitochondrial translation elongation factor Ts), lonp 1 (mitochondrial Lon protease), spg 7 (paraplegin), oma 1 (mitochondrial metalloendopeptidase OMA1), clpB (mitochondrial caseinolytic matrix peptidase chaperone subunit B), atp 23 (mitochondrial inner membrane protease ATP23), twnk (mitochondrial twinkle mtDNA helicase); and for mitophagy - mieap (encoding mitochondrial eating protein), hyou 1 (hypoxia upregulated protein 1), prkn (parkin), pink 1 (PTEN- induced kinase 1), and pgam 5 (mitochondrial serine/threonine protein phosphatase PGAM5). The revealed species-specific differences in the expression of the mitochondrial quality control pathways shed light on the potentially important mechanisms of mitochondrial protection against H/R-induced damage that might contribute to hypoxia tolerance in marine bivalves. : {"references": ["Steffen, J. B. M., Falfushynska, H. I., Piontkivska, H. and Sokolova, I. M. (2020). Molecular Biomarkers of the Mitochondrial Quality Control Are Differently Affected by Hypoxia-Reoxygenation Stress in Marine Bivalves Crassostrea gigas and Mytilus edulis. Frontiers in Marine Science 7."]}