A rapid PCR-based test for identification of fifteen British bat species ...

(Uploaded by Plazi for the Bat Literature Project) The development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leis...

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Bibliographic Details
Main Authors: Hamilton, P. B., Uren Webster, T. M., Basiewicz, M., Kennedy, E. V., De-Bastos, E. S. R., Mathews, F.
Format: Article in Journal/Newspaper
Language:unknown
Published: Zenodo 2015
Subjects:
Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
Barbastella barbastellus
Myotis nattereri
Nyctalus noctula
Pipistrellus nathusii
Pipistrellus pipistrellus
Online Access:https://dx.doi.org/10.5281/zenodo.13467174
https://zenodo.org/doi/10.5281/zenodo.13467174
Description
Summary:(Uploaded by Plazi for the Bat Literature Project) The development of a rapid method for identification of the fifteen common species of British bat is described: Barbastella barbastellus, Eptesicus serotinus, Myotis bechsteinii, Myotis daubentonii, Myotis mystacinus, Myotis nattereri, Nyctalus leisleri, Nyctalus noctula, Plecotus auritus, Plecotus austriacus, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Rhinolophus ferrumequinum and Rhinolophus hipposideros. A single multiplex PCR containing five fluorescently-tagged primers and fifteen species-specific primers targeting the mitochondrial control region and genes for cytb and ND1 produces clear peaks that are unique to each species when visualised on a DNA sequencer. The multiplex also includes primers to test for PCR-amplifiable bat DNA. This method will facilitate high throughput species identification of British bats, particularly in cases where phenotypic identification is difficult. ...

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