Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ...
(Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested....
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ftdatacite:10.5281/zenodo.13445497 2024-09-30T14:45:45+00:00 Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... Lelli, D. Moreno, A. Lavazza, A. Bresaola, M. Canelli, E. Boniotti, M. B. Cordioli, P. 2013 https://dx.doi.org/10.5281/zenodo.13445497 https://zenodo.org/doi/10.5281/zenodo.13445497 unknown Zenodo hash://md5/3b0b154bc4f18ae262e003969d375b41 hash://sha256/c523a9762e5bc4ca0505e9831153158e63afbbde00ea638c2e0c3c05b64a0840 zotero://select/groups/5435545/items/RTJSAW4M https://zotero.org/groups/5435545/items/RTJSAW4M https://linker.bio/cut:hash://md5/1bce88fd3c1ad6e3be536ca5bb3ff778!/b278226-280686 hash://md5/26f7ce5dd404e33c6570edd4ba250d20 hash://md5/3b0b154bc4f18ae262e003969d375b41 hash://sha256/c523a9762e5bc4ca0505e9831153158e63afbbde00ea638c2e0c3c05b64a0840 zotero://select/groups/5435545/items/RTJSAW4M https://zotero.org/groups/5435545/items/RTJSAW4M https://linker.bio/cut:hash://md5/1bce88fd3c1ad6e3be536ca5bb3ff778!/b278226-280686 hash://md5/26f7ce5dd404e33c6570edd4ba250d20 https://dx.doi.org/10.5281/zenodo.1410543 https://dx.doi.org/10.5281/zenodo.13445496 Biodiversity Mammalia Chiroptera Chordata Animalia bats bat JournalArticle ScholarlyArticle article-journal 2013 ftdatacite https://doi.org/10.5281/zenodo.1344549710.5281/zenodo.141054310.5281/zenodo.13445496 2024-09-02T10:15:12Z (Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT-PCR assays targeting the L1 gene. Through a multiplex RTPCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference ... Article in Journal/Newspaper Vespertilio murinus DataCite |
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Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
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Biodiversity Mammalia Chiroptera Chordata Animalia bats bat Lelli, D. Moreno, A. Lavazza, A. Bresaola, M. Canelli, E. Boniotti, M. B. Cordioli, P. Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
topic_facet |
Biodiversity Mammalia Chiroptera Chordata Animalia bats bat |
description |
(Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT-PCR assays targeting the L1 gene. Through a multiplex RTPCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference ... |
format |
Article in Journal/Newspaper |
author |
Lelli, D. Moreno, A. Lavazza, A. Bresaola, M. Canelli, E. Boniotti, M. B. Cordioli, P. |
author_facet |
Lelli, D. Moreno, A. Lavazza, A. Bresaola, M. Canelli, E. Boniotti, M. B. Cordioli, P. |
author_sort |
Lelli, D. |
title |
Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
title_short |
Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
title_full |
Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
title_fullStr |
Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
title_full_unstemmed |
Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ... |
title_sort |
identification of mammalian orthoreovirus type 3 in italian bats ... |
publisher |
Zenodo |
publishDate |
2013 |
url |
https://dx.doi.org/10.5281/zenodo.13445497 https://zenodo.org/doi/10.5281/zenodo.13445497 |
genre |
Vespertilio murinus |
genre_facet |
Vespertilio murinus |
op_relation |
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op_doi |
https://doi.org/10.5281/zenodo.1344549710.5281/zenodo.141054310.5281/zenodo.13445496 |
_version_ |
1811646210675572736 |