Identification of Mammalian Orthoreovirus Type 3 in Italian Bats ...

(Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested....

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Bibliographic Details
Main Authors: Lelli, D., Moreno, A., Lavazza, A., Bresaola, M., Canelli, E., Boniotti, M. B., Cordioli, P.
Format: Article in Journal/Newspaper
Language:unknown
Published: Zenodo 2013
Subjects:
Biodiversity
Mammalia
Chiroptera
Chordata
Animalia
bats
bat
Vespertilio murinus
Online Access:https://dx.doi.org/10.5281/zenodo.13445497
https://zenodo.org/doi/10.5281/zenodo.13445497
Description
Summary:(Uploaded by Plazi for the Bat Literature Project) This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples and dead individuals available from rehabilitation centres or collected from known roost sites were virologically tested. In total, 112 carcasses of bats found dead, and 44 faecal samples were analysed. Nineteen viral strains were isolated by in vitro cell culture from faecal and tissue samples of different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros and Vespertilio murinus), and they were morphologically identified as reoviruses by negative staining electron microscopy observation. The definitive assignment of all isolates to MRV was confirmed by RT-PCR assays targeting the L1 gene. Through a multiplex RTPCR assay targeting the S1 gene, we typed 15 of 19 isolates as MRV type 3. Partial L1 (416 bp) and complete S1 (1416 bp) sequences of the isolates were analysed and compared with those of reference ...

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