Intestinal Tropism of a Betacoronavirus (Merbecovirus)in Nathusius's Pipistrelle Bat (Pipistrellus nathusii), Its Natural Host ...

• Main Authors: Mols, Vera C., Lamers, Mart M., Leijten, Lonneke Me., Breugem, Tim I., Van De Bildt, Marco Wg., Van Den Doel, Petra B., Lina, Peter Hc., Koopmans, Marion Pg., Haagmans, Bart L., Kuiken, Thijs, Begeman, Lineke, Gallagher, Tom
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Zenodo 2023
• Subjects: Biodiversity; Mammalia; Chiroptera; Chordata; Animalia; bats; bat; Pipistrellus nathusii
• Online Access: https://dx.doi.org/10.5281/zenodo.13441955; https://zenodo.org/doi/10.5281/zenodo.13441955

(Uploaded by Plazi for the Bat Literature Project) The emergence of several bat coronavirus-related disease outbreaks in human and domestic animals has fueled surveillance of coronaviruses in bats worldwide. However, little is known about how these viruses interact with their natural hosts. We demonstrate a Betacoronavirus (subgenus Merbecovirus), PN-bCoV, in the intestine of its natural host, Nathusius's Pipistrelle Bat (Pipistrellus nathusii), by combining molecular and microscopy techniques. Eighty-eight P. nathusii bat carcasses were tested for PN-bCoV RNA by RT-qPCR, of which 25 bats (28%) tested positive. PN-bCoV RNA was more often detected in samples of the intestinal tract than in other sample types. In addition, viral RNA loads were higher in intestinal samples compared to other sample types, both on average and in each individual bat. In one bat, we demonstrated Merbecovirus antigen and PN-bCoV RNA expression in intestinal epithelium and the underlying connective tissue using immunohistochemistry ...