Biochemical Alteration Of Triclosan And The Productive Role Of Garlic Extract And Vitamin C In Zebrafish (Branchydanio Rerio)

Zebrafish has been a prominent model vertebrate in a variety of biological discipline. Sustainable information gathered from developmental and genetic research, together with mear completion of the zebrafish genome project, has placed zebrafish in an attractive position for use as a toxicological mo...

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Main Author: D. Nagarajan
Format: Text
Language:unknown
Published: Zenodo 2017
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Online Access:https://dx.doi.org/10.5281/zenodo.1312671
https://zenodo.org/record/1312671
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Summary:Zebrafish has been a prominent model vertebrate in a variety of biological discipline. Sustainable information gathered from developmental and genetic research, together with mear completion of the zebrafish genome project, has placed zebrafish in an attractive position for use as a toxicological model. There is a clear potential for Branchydanio rerio to provide valuable new sights into chemical toxicity, drug discovery and human diseases using recent advances in forward and reverse genetic techniques. The present study was carried out the impact on exposure of sub lethal concentration of triclosan induced biochemical parameters. This experiment was carried out from 7th day to 28th days of the exposure of triclosan, triclosan with garlic extract, triclosan with vitamin C, garlic extract, vitamin C and maintained with control. Triclosan of 0.32g, 1ml of garlic extract and 1g of vitamin C were used in this study. The result proved that garlic extract and vitamin C were played a productive role in treated with triclosan of zebrafish and also enhances the biochemical and enzymological parameters, and it act as detoxifying agent of triclosan. This study shows the changes in biochemical parameters of blood, which has been recovering by garlic extract and vitamin C against triclosan exposed zebrafish. : {"references": ["Blazer, D.G., 1992. Religion coping and depression among elderly hospitalized medically ill man. Am. J. Psych., 149, 693-1700.", "Buchal, M. and Langdon, C., 1998. Evaluation of lipid spray beads for the delivery of water soluble materials to a marine suspension-feeder, the manila clam tapes philippinarum (Deshayes 1853). Aquac. Nutr. 4, 265\u2013284.", "Dabrowski, K., El-Fiky, N., K\u00f6ch, G., Frigg, M. and Wieser, W., 1990 . Requirement and utilization of AA and ascorbic acid sulphate in juvenile rainbow trout. Aquaculture, 91, 317-37.", "Daughton, C.G. and Ternes, T.A., 1999. Pharmaceuticals and personal care products in the environment: agents of subtle change? Environ. Health Perspect., 107, 907-938.", "Das, B.K. and Mukherjee, S.C., 2002. Toxicity of cypermethrin in Labeo rohita fingerlings: biochemical,enzymatic and haematological consequences. Comp Biochem. Physiol., 134, 109-121.", "El-Ashker, M., Abdelhamid, F., Risha, E., Salama, M., El-Sebaei, M., 2015. Vitamin C Ameliorates Gentamicin-Induced Acute Kidney Injury in Equines: An experimental study. J. Equ. Vet. Sci., 35, 238-243.", "Eo, J. and Lee, K.J., 2008. Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish. Shellfish Immunol., 25 (5), 611-616.", "Folch, J., Lees, M. and Sloane Stanley, G., 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226(1), 497-509.", "Gabriele, L. Pedroso, Thais O. Hammes, Thayssa D.C. Escobar, Laisa B. Fracasso, Luiz Felipe Forgiarini and Themis R. da Silveira, 2012. Blood collection for biochemical analysis in adult zebrafish. J. Vis. Exp., (63), 3865. DOI: 10.3791/3865.", "Groppo, F.C., Ramacciato, J.C., Simoes, R.P., Florio, F.M. and Sartoratto, A., 2002. Antimicrobial activity of garlic, tea tree oil and chlorhexidine against oral microorganisms. Int. Dent. J., 52, 433-437.", "Hahn, G., 1996. In: Koch, H.P., Lawson, L.D., eds. Garlic: the science and therapeutic application of Allium sativum L and related species (2nd Edn). Baltimore Williams and Wilkins, pp. 1-24.", "Halden, R.U. and Paull, D.H., 2005. Co-occurrence of triclocarban triclosan in U.S. water resources. Environ. Sci. Technol., 39(6), 1420-1426.", "Ibrahem, M.D., Fathi, M., Mesalhy, S. and El-Aty, A.A., 2010. Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity, and resistance of Nile tilapia (Oreochromis niloticus. Fish Shellfish Immunol., 29, 241-246.", "Ibrahem, M.D., Fathi, M., Mesalhy, S. and El-Aty, A.A., 2010. Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity, and resistance of Nile tilapia (Oreochromis niloticus. Fish Shellfish Immunol., 29, 241-246.", "Li, Y. and Lovell, R.T., 1985. Elevated levels of dietary ascorbic acid increase immune response in channel catfish. J. Nutr., 115, 123-131.", "Li, Y. and Schellhorn, H.E., 2007. New Developments and Novel Therapeutic Perspectives for Vitamin C. J. Nutr., 137, 2171-2184.", "Mansell, P. and Reckless, J., 1991. Effects on serum lipids, blood pressure, coagulation, platelet aggregation and vasodilation. B.M.J., 303, 379-380.", "Masumoto, T., Hidetuyo, H. and Shimeno, S., 1991. Ascorbic acid's role in aquaculture nutrition. In: Proceedings of the aquaculture feed processing and nutrition Workshop. Indonesia: Am. Soybean Assoc., p. 42- 48.", "Nordgreen, J., Garner, J.P., Janczak, A.M., Ranheim, B., Muir, W.M. and Horsberg, T.E., 2009. Thermonociception in fish: effects of two different doses of morphine on thermal threshold and posttest behaviour in goldfish (Carassius auratus). App. Ani. Behav. Sci., 119, 101-107.", "Onal, U. and Langdon, C.J., 2004. Characterization of lipid spray beads for delivery of Glycine and tyrosine to early marine fish larvae. Aquaculture, 233, 495-511.", "Ozkanlar, S. and Akcay, F., 2002. Antioxidant vitamins in atherosclerosis-animal experiments and clinical studies. Adv. Clin. Exp. Med., 21,115-123.", "Sandel, L.J. and Daniel, J.C., 1988. Effect of ascorbic acid on RNA levels in short term chondrocyte culture. Connect Tissue Res., 17(1), 11-22.", "Soliman, A.K., Jauncey, K,. Roberts, R.J. 1986. The effect of varying forms of dietary ascorbic acid on the nutrition of juvenile tilapias (Oreochromis niloticus). Aquaculture, 52, 1- 10.", "Spitsbergen, J.M. and Kent, M.L., 2003. The state of the art of the zebrafish model for toxicology and toxicologic pathology research\u2013advantages and current limitations. Toxicol Pathol., 31, 62\u201387.", "Teraoka, H., Dong, W. and Hiraga, T., 2003. Zebrafish as a Novel Experimentalmodel for Developmental Toxicology. Congenit. Anom. (Kyoto), 43, 123-132.", "Tewary A. and Patra, B.C., 2008. Use of vitamin C as an immunostimulant. Effect on growth, nutritional quality, and immune response of Labeo rohita (Ham.). Fish. Physiol. Biochem., 34(3), 251-259.", "Varley Harold, 1980. Practical Clinical Biochemical William Heineman Medical Books Ltd., New Yark.", "Xie, Z., Niu, C., Zhang, Z. and Bao, L., 2006. Dietary ascorbic acid may be necessary for enhancing the immune response in Siberian sturgeon (Acipenser baerii), a species capable of ascorbic acid biosynthesis. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 45 (2), 152-157.", "Yamamoto, Y., Sato, M., Ikeda, S. 1985. Existence of L-gulonolactone oxidase in some teleosts. Bull. Jpn. Soc., Sci. Fish., 44, 775-779."]}