Molecular-Genetic Identification In Forestry With Using Of Genomic Technologies

• Main Authors: Boronnikova, S., Kalendar, R., Ya., Prishnivskaya, Yu., Vasileva, Nassonova, E., Krasilnikov, V.
• Format: Text
• Language: Russian
• Published: Zenodo 2018
• Subjects: Molecular-genetic identification; ISSR-PCR marker; barcode; P. sylvestris; karelian
• Online Access: https://dx.doi.org/10.5281/zenodo.1312149; https://zenodo.org/record/1312149

Molecular-genetic analysis and identification of six populations of Pinus sylvestris L. located in East of Russian plain with using of ISSR-method DNA polymorphism analysis were held. 135 ISSP-PCR markers of six researched P. sylvestris populations was identified, 128 of which were polymorphic (P95= 0.948). In researched populations, 9 rare ISSR-PCR markers were identified. Identification monomorphic species and polymorphic ISSR-PCR markers and their combinations for molecular-genetic identification of researched populations were detected. Molecular-genetic formulas and barcodes of six populations of P. sylvestris were prepared. : {"references": ["Vetchinnikova, L. V., Titov, A. F., & Kuznetsova, T. Yu. (2013). Karelian birch: biological features, resource dynamics and reproduction. Petrozavodsk: Karelian Research Center of the Russian Academy of Sciences, 312.", "Boronnikova, S. V. (2013). Molecular genetic analysis and assessment of the state of gene pools of resource species of plants in the Perm Krai. Perm: Perm. state. nat. Issled. un-t. 223.", "Kleinhof, I. A. (2008). Global aspects of the development of the forest sector of the economy. Forest Herald, (5). 115-119.", "Rogers, S. O., & Bendich, A. J. (1985). Extraction of DNA from milligram quantities of fresh, herbarium and mummified plant tissues. Plant Molecular Biology, l (19). 69-76.", "Zietkiewicz, E. Rafalski, A., & Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, (20). 176-183.", "Boronnikova, S. V., & Calendar, R. N. (2010). Using the IRAP-method for analysis of genetic variability of populations of resource and rare plant species. Genetika, 46 (1). 44-50.", "Pat. 2012119341 Russian Federation A01H1/00. Method for molecular genetic identification of populations of woody plant species. Boronnikova, S. V., & Boboshina, I. V., applicant and patent holder Federal State Budget Educational Institution of Higher Professional Education \"Perm State National Research University\" \u21162012119341; Application 11.05.2012; publ. 10/02/2014."]}