Effect Of Abscisic Acid On Depositing Of The Karelian Birch In Vitro

• Main Author: Kontsevaya, I.
• Format: Text
• Language: Russian
• Published: Zenodo 2018
• Subjects: abscisic acid; regenerative capacity; culture in vitro; birch; depositing; karelian
• Online Access: https://dx.doi.org/10.5281/zenodo.1312144; https://zenodo.org/record/1312144

The possibility of prolonged continuous cultivation in vitro of explants of Karelian birch on media supplemented with abscisic acid (including those with a high content of sucrose, activated carbon, 6-BAP) is discussed in the paper. Methods: culture in vitro; statistical analysis. Periodic visual monitoring of the condition of micro-plants and (on occasion) transplanting plants to a fresh nutrient medium without hormones are necessary during depositing the Karelian birch in vitro. There is no negative effect of abscisic acid on the growth and development of birch explants sub-cultured on fresh media after different storage times (including cases with additional high contents of sucrose, activated carbon and 6-BAP to the nutrient medium). : Работа выполнена при поддержке ГПНИ (№ темы М16-33). : {"references": ["Reed, B.M. (2007). Cryopreservation of in vitro tissue of deciduous forest trees // Plant Cryopreservation: A Practical Guide. New York: Springer. 365-386.", "Koval, S. F., Koval, V. S., Tymchuk, S. M., & Boguslavsky, R. L. (2003). Genetic collections: problems of formation, preservation and use. Cytology and Genetics, 37 (4), 46-53.", "Chetverikova, E. P. (1999). The role of abscisic acid in the frost resistance of plants and cryopreservation of cultures in vitro. Plant physiology, 46 (6), 823-829.", "Jarret, R. L., & Gawel, N. (1991). Abscisic acid-induced growth inhibition of sweet potato (Ipomoea batatas L.) in vitro. Plant Cell, Tissue and Organ Culture, 24(1), 13-18.", "Sidorovich, E. A, & Kutas, EN (1996). Clonal micropropagation of new fruit and berry plants. Minsk: Navuka i tehnika. 249.", "Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture., 30, 421-427.", "Belokurova, V. B., Listvyan, E. V., Listvyan, E. V., Sikura, J. J., Gleb, Yu. Yu., & Kuchuk, N. V. (2005). Use of plant biotechnology methods to preserve and study the biodiversity of the world's flora. Cytology and Genetics, (1). 41-51.", "Mashkina, O. S., & Tabatskaya, T. M. (2005). Recommendations for the preservation and reproduction by methods of biotechnology of valuable genotypes of Karelian birch, aspen, white and gray poplar. 29."]}