Influence Of Cyperus Rotundus Active Principles Inhibit Viral Multiplication And Stimulate Immune System In Indian White Shrimp Fenneropenaeus Indicus Against White Spot Syndrome Virus Infection

• Main Authors: T. Citarasu, M. Michaelbabu V. N. Vakharia
• Format: Text
• Language: English
• Published: Zenodo 2015
• Subjects: Antiviral drugs; Cyperus rotundus; Fenneropenaeus indicus; WSSV.; Austin; Indian; Kondo; Levy; MacRae; Marian; Homarus gammarus
• Online Access: https://dx.doi.org/10.5281/zenodo.1099901; https://zenodo.org/record/1099901

The rhizome of Java grass, Cyperus rotundus was extracted different organic polar and non-polar solvents and performed the in vitro antiviral and immunostimulant activities against White Spot Syndrome Virus (WSSV) and Vibrio harveyi respectively. Based on the initial screening the ethyl acetate extract of C. rotundus was strong activities and further it was purified through silica column chromatography and the fractions were screened again for antiviral and immunostimulant activity. Among the different fractions screened against the WSSV and V. harveyi, the fractions, FIII to FV had strong activities. In order to study the in vivo influence of C. rotundus, the fractions (F-III to FV) were pooled and delivered to the F. indicus through artificial feed for 30 days. After the feeding trail the experimental and control diet fed F. indicus were challenged with virulent WSSV and studied the survival, molecular diagnosis, biochemical, haematological, and immunological parameters. Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets helped to significantly (P<0.01) suppressed viral multiplication, showed significant (P<0.01) differences in protein and glucose levels, improved total haemocyte count (THC), coagulase activity, significantly increased (P <= 0.001) prophenol oxidase and intracellular superoxide anion production compared to the control shrimps. Based on the results, C. rotundus extracts effectively suppressed WSSV multiplication and improve the immune system in F. indicus against WSSV infection and this knowledge will helps to develop novel drugs from C. rotundus against WSSV. : {"references": ["T. Citarasu, R. R. J. Sekar, M. M. Babu and M.P. Marian, \"Developing\nArtemia enriched herbal diet for producing quality larvae in Penaeus\nmonodon.\" Asian Fisheries Science, vol 15, 2002, pp. 21-32.", "FAO, \"Report of the expert consultation on the proposed subcommittee\non aquaculture of the committee on fisheries\" Rome, FAO, 2000, p. 36.", "T. Flegel, W. S. Boonyaratpalin and B. Withyachumnarnkul, \"Progress\nin research on yellow-head virus and white spot virus in Thailand\" In:\nAsian Fisheries Society (Eds. T.W Flegel, I.H. MacRae), Manila, 1997.\npp. 285-96.", "T. Citarasu, K.V. Ramalingam, R.R.J. Sekar, M. M. Babu and M.P.\nMarian, \"Influence of the antibacterial herbs, Solanum trilobatum,\nAndrographis paniculata and Psoralea corylifolia on the survival,\ngrowth and bacterial load of Penaeus monodon post larvae.\"\nAquaculture International, vol 11, 2003, pp.583-95.", "T. Citarasu, V. Sivaram, G. Immanuel, N. Rout and V. Murugan,\n\"Influence of selected Indian immunostimulant herbs against white spot\nsyndrome virus (WSSV) infection in black tiger shrimp, Penaeus\nmonodon with reference to haematological, biochemical and\nimmunological changes.\" Fish and Shellfish Immunology, vol. 21, 2006,\npp.372-84.", "T. Citarasu, \"Natural antimicrobials.\" In: Aquaculture infective disease\nin aquaculture: prevention and control.(Eds. B. Austin) Woodhead\nPublishing Ltd, United Kingdom, 2012. p. 419.", "P. Smith, M. P. Hiney and O. B. Samuelsen, \"Bacterial resistance to\nantimicrobial agent used in fish farming: a critical evaluation of method\nand meaning.\" Annual Review of Fish Diseases, vol 4, 1994, pp. 273-\n313.", "T. Citarasu, \"Herbal biomedicines - a new opportunity to aquaculture\nindustry.\" Aquaculture International, vol 18, 2010, pp. 403-14.", "G. Balasubramanian, M. Sarathi, S. Rajesh Kumar and A.S. Sahul\nHameed. \"Screening the antiviral activity of Indian medicinal plants\nagainst white spot syndrome virus in shrimp.\" Aquaculture, vol\n263,2007, pp. 15-9.\n[10] P. Raja Rajeswari, S. Velmurugan, M. M. Babu, K. Kesavan and T.\nCitarasu, \"A study on the influence of selected Indian herbal active\nprinciples on enhancing the immune system in Fenneropenaeus indicus\nagainst Vibrio harveyi infection.\" Aquaculture International, vol 20\n2012, pp. 1009\u20131020.\n[11] M. Boonyaratpalin, \"Nutritional requirements of grouper Epinephelus.\"\nIn: The proceedings of grouper culture. Thailand: National Institute of\nCoastal Aquaculture. Department of Fisheries, 1993. p. 50-55.\n[12] R. Namita, S. Kumar, S. Jaganmohan and V. Murugan, \"DNA vaccines\nencoding viral envelope proteins confer protective immunity against\nWSSV in black tiger shrimp.\" Vaccine, vol 25, 2007, pp. 2778-86.\n[13] C.P. Malik, M.B.Singh, \"Plant enzymology and histoenzymology.\"\nKalyani Publishers, New Delhi, 1980, p. 278.\n[14] K. N. Sachdev, \"Clinical pathology and bacteriology.\" Jaypee Brother,\nNew Delhi, 1983, p. 133.\n[15] G. Le Moullac, M. Le Groumellec, D. Ansquer, S. Froissard, P.\nAquacop Levy, \"Haematological and phenoloxidase activity changes in\nthe shrimp Penaeus stylirostris in relation with the moult cycle:\nprotection against vibriosis.\" Fish and Shellfish Immunology, vol 7,\n1997, pp.227-34.\n[16] L. Hagerman, \"Haemocyanin concentration of juvenile lobster\n(Homarus gammarus) in relation to molting cycle and feeding\nconditions.\" Marine Biology, vol 77, 1983, pp. 11-17.\n[17] K. S\u00f6derh\u00e4ll and V.J. Smith, \"Separation of haemocyte populations of\nCarcinus maenus and other marine decapods and phenoloxidase\ndistribution.\" Developmental and Comparative Immunology, vol 7,\n1983, PP. 229\u2013239\n[18] Y.L. Song and Y.T. Hsieh, \"Immunostimulation of tiger shrimp\n(Penaeus monodon) haemocytes for generation of microbiocidal\nsubstances: analysis of reactive oxygen species.\" Developmental and\nComparative Immunology, vol 18, 1994, pp. 201-209.\n[19] S. Direkbusarakom, A. Herunsalee, S. Boonyaratpalin, Y. Danayadol\nand U. Aekpanithanpong, \"Effect of Phyllanthus spp against yellow\nhead baculovirus infection in black tiger shrimp, Penaeus monodon.\" In:\nDisease in Asian aquaculture II. (Eds. M. Shariff, J.R. Arthur, R.P.\nSubasinghe), Fish Health Section, Asian Fisheries Society, Manila,\n1993. p. 81-88.\n[20] M. Minomol, \"Culture of Gold fish Carassius auratus using medicinal\nplants having immunostimulant characteristics.\" M. Phil Dissertation,\nM. S. University, Tirunelveli, India, 2005\n[21] A. Yogeeswaran, S. Velmurugan, S.M.J. Punitha, M.M. Babu, T.\nSelvaraj, T. Kumaran and T. Citarasu, \"Protection of Penaeus monodon\nagainst white spot syndrome virus by inactivated vaccine with herbal\nimmunostimulants.\" Fish and Shellfish Immunology, vol 32(6), 2012,\npp. 1058-67.\n[22] C.F. Lo, C.H. Ho, C.H.Chen, K.F. Liu, Y.L. Chiu, P.Y. Yeh, \"Detection\nand tissue tropism of white spot syndrome baculovirus (WSBV) in\ncaptured brooders of Penaeus monodon with a special emphasis on\nreproductive organ.\" Disease in Aquatic Organism, vol 30, 1997, pp. 53-\n72.\n[23] M.R. Hall, E.H and Van Ham, \"The effect of different types of stress on\nblood glucose in the giant tiger prawn Penaeus monodon.\" Journal of\nWorld Aquaculture Society, 29, 1998, 290- 9.\n[24] M. Maeda, T. Itami, M. Kondo, O. Henning, Y. Takahashi and I.\nHirono, \"Characteristics of penaeid rod shaped DNA virus of kuruma\nshrimp.\" In: New approaches to viral disease of aquatic animals, NRIA\nInternational Workshop. Nansei, Mie, Japan: National Research Institute\nof Aquaculture, 1997, p. 218-28.\n[25] Yoganandhan K, Thirupathi S and Sahul Hameed AS. Biochemical,\nphysiological and hematological changes in white spot syndrome virus\ninfected shrimp, Penaeus indicus. Aquaculture 2002;62:1e11.\n[26] F. Bindhu, S. Velmurugan, M. B. S. Donio, M. Michaelbabu and T.\nCitarasu, \"Influence of Agathi grandiflora active principles inhibit viral\nmultiplication and stimulate immune system in Indian white shrimp\nFenneropenaeus indicus against white spot syndrome virus infection.\"\nFish and Shellfish Immunology, vol 41, 2014, pp. 482-492\n[27] M.W. Johansson, P. Keyser, K. Sritunyalucksana and K. Soderhall,\n\"Crustacean haemocytes and haematopoiesis.\" Aquaculture, VOL 191,\n2000, pp. 45-52."]}