Bioprocessing Of Proximally Analyzed Wheat Straw For Enhanced Cellulase Production Through Process Optimization With Trichodermaviride Under Ssf

• Main Authors: Ishtiaq Ahmed, Zia, Muhammad Anjum, Iqbal, Hafiz Muhammad Nasir
• Format: Text
• Language: English
• Published: Zenodo 2010
• Subjects: Cellulase; Lignocellulosic residue; Processoptimization; Proximal analysis; SSF; Trichoderma viride.; Duarte; DuBois; Eklund; Fang; Lowry; Macleod; McIntosh; Pedersen; Randall; Rodriguez; Romero; Turunen; Wyman; sami
• Online Access: https://dx.doi.org/10.5281/zenodo.1083051; https://zenodo.org/record/1083051

The purpose of the present work was to study the production and process parameters optimization for the synthesis of cellulase from Trichoderma viride in solid state fermentation (SSF) using an agricultural wheat straw as substrates; as fungal conversion of lignocellulosic biomass for cellulase production is one among the major increasing demand for various biotechnological applications. An optimization of process parameters is a necessary step to get higher yield of product. Several kinetic parameters like pretreatment, extraction solvent, substrate concentration, initial moisture content, pH, incubation temperature and inoculum size were optimized for enhanced production of third most demanded industrially important cellulase. The maximum cellulase enzyme activity 398.10±2.43 μM/mL/min was achieved when proximally analyzed lignocellulosic substrate wheat straw inocubated at 2% HCl as pretreatment tool along with distilled water as extraction solvent, 3% substrate concentration 40% moisture content with optimum pH 5.5 at 45°C incubation temperature and 10% inoculum size. : {"references": ["M. C. Chang, \"Harnessing energy from plant biomass,\" Curr. Opin.\nChem. Biol., vol. 11, pp. 677-684, 2007.", "D. Damisa, J. B. Ameh, and V. J. Umoh, \"Effect of chemical\npretreatment of some lignocellulosic wastes on the recovery of cellulase\nfrom Aspergillus niger AH3 mutant,\" Afr. J. Biotechnol., vol. 7, no. 14,\npp. 2444-2450, 2008.", "L. R. Lynd, R. T. Elander, and C. E. Wyman, \"Likely features and costs\nof mature biomass ethanol technology,\" Appl. Biochem. Biotechnol., vol.\n57-58, pp. 741-761, 1996.", "M. Raimbault, \"General and microbiological aspects of solid substrate\nfermentation,\" Elect. J. 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